Cat #: 29022-1
Size: 1mg
Brand: Biotium
Wheat Germ Agglutinin Lectin (WGA) marked with CF or HRP dyes from Biotium. It is a carbohydrate-binding lectin that has a high affinity for sialic acid and N-acetylglucosamine and stains the scars of yeast buds, as well as the cell membrane of gram + bacteria and mammalian cells.
Product attributes
Cell localization probe: Membrane/cell surface
For fixed cells, for live/intact cells
Cell permeability: Waterproof membrane
Fixing options
Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)
Colors
Blue, green, orange, red, far-red, near-infrared
Product description
Wheat germ agglutinin (WGA) is a carbohydrate-binding lectin that has a high affinity for sialic acid and the N-acetylglucosamine moieties of glycoproteins. As a result, the WGA conjugates will mark the scars of yeast buds and the cell membrane of gram + bacteria and mammalian cells.
- Stain yeast bud scars, as well as the cell membrane of gram + bacteria and mammalian cells or tissues.
- Suitable for Western Blot, ELISA, immunohistochemistry, and other standard immunoassay applications
- A retrograde or antegrade neural tracer
- Resists fixation and permeabilization
- Choice of 13 CF® tints from UV to Near-Infrared or HRP
- Superior CF® dyes are glossy, photostable, and soluble in water
WGA is commonly used to label glycoproteins for plasma membrane imaging in living or fixed cells, for staining tissue sections, or for Western blotting. WGA can be used as a Gram stain to fluorescently label gram + bacteria but not gram bacteria. WGA also binds to bud scars in budding yeasts such as Saccharomyces cerevisiae.
Find the right tint for your application
WGA and other lectins are carbohydrate-binding proteins that recognize specific sugar residues on glycoproteins. The presence and distribution of these targets vary between cell and tissue types. As a result, other cell surface stains or other lectin conjugates, concanavalin A conjugates (Con A) and PNA lectin conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of living cells and resist binding and permeabilization.
When cells are fixed and permeabilized prior to staining, fluorescent lectins stain both the cell surface and organelles in the secretory pathway. Lectins can be toxic or stimulant to living cells depending on the cell type. To find the right stain for your application, see our Cell Surface and Membrane Stain Comparison or download our Surface and Membrane Stain Brochure. See our Cell Stain Chart for more information on how our dyes stain various organisms.