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| Assay type | Sandwich-ELISA |
| Format | 96T/48T |
| Assay time | 2.5h |
| Detection range | 0.13-8 ng/mL |
| Sensitivity | 0.06 ng/mL |
| Sample type & Sample volume | serum, plasma, urine; 50μL |
| Specificity | This kit recognizes Human NGAL in samples. No significant or interference between Human NGAL and analogs was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Samples (or Standards) and biotinylated detection antibodies specific for Human NGAL are added to the micro ELISA plate wells. Human NGAL would combine with the specific antibody.
Then Avidin-Horseradish Peroxidase (HRP) conjugate is added successively to each microplate well and incubated. Free components are washed away. The substrate solution is added to each well.
Only those wells that contain Human NGAL, biotinylated detection antibodies, and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow.
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the OD of the samples to the standard curve.
Kit components & Storage
An unopened kit can be stored at 2-8℃ for six months. After the test, the unused wells and reagents should be stored according to the table below.