Arthrogen-CIA 5-Clone Cocktail Kit

Chondrex Inc’s. all around referred to Collagen Antibody-Induced Arthritis (CAIA) immunizer mixed drink was initially presented as a 4-clone neutralizer mixed drink pack in 1998 (Arthrogen-CIA arthritogenic monoclonal antibodies). Be that as it may, to advance reliable enlistment of the CAIA model, Chondrex, Inc. has added a fifth immunizer clone to our unique mixed drink to make the Arthrogen-CIA 5-Clone Cocktail pack. This better 5-clone mixed drink considers joint pain enlistment in CAIA-low responder strains like C57BL/6 mice and hereditarily adjusted mice with C57BL/6 (H-2b) or 129/Sv (H-2b) foundations.

Contents

• Benefits of CAIA
• CAIA versus CIA
• Cost and Time Comparison
• Unique 4-clone Cocktail
• Recently Improved 5-Clone Cocktail
• Arthritogenecity of Chondrex 5-Clone Cocktail versus Contender
• Significant Roles of Bacterial Toxins in the Pathogenesis of Autoimmune Arthritis
• Mouse Strains
• Two Factors to Consider for Users of Animal Models
• Arthritogenic Epitopes

Benefits of CAIA

1) Faster Results: Severe joint pain is prompted inside 24-48 hours (Figure 1A) rather than the a month expected to actuate CIA after vaccination with type II collagen (Figure 1B).
2) Broad Applicability: Unlike the CIA model, which requires a limited MHC haplotype, the CAIA model can be prompted in practically all strains of mice, including CIA-safe, Tcell insufficient, quality knockout, and transgenic mice.
3) Consistency: The seriousness of joint pain clearly corresponds to the portion of monoclonal immune response mixed drink, and more extreme and reliable joint inflammation can be prompted by expanding the portion of monoclonal immunizer mixed drink.
Accordingly, this model is great for concentrating on the obsessive job of individual quality items, cytokines, as well as screening and assessing mitigating specialists (Figure 1) without impacts of complete or inadequate Freund’s adjuvant, which emphatically influences the host’s insusceptible framework.

Figure 1 – Comparison of true CIA and monoclonal immune response LPS initiated joint pain (CAIA)
(A) CAIA: A mixed drink of 5 monoclonal antibodies to type II collagen was infused IP on day 0 followed by an
IP infusion of LPS (50 µg) on day 3. Joint pain created on day 4 and arrived at its crested on days 7-8.
The helpful impacts of test not entirely set in stone on day 7. Osteoclast arrangement and bone
corruption will keep on advancing until day 21 (not shown).
(B) Authentic CIA: Arthritis created around a month after vaccination, and arrived at its first top after 7
weeks, and afterward a second top following 9-10 weeks. It takes more time to about two months to assess the restorative impacts of
test compounds.

Unique 4-Clone Cocktail

Collagen-prompted joint pain (CIA) is interceded via autoantibodies to type II collagen. A comparative joint pain can be prompted by a 4-clone mixed drink of against type II collagen monoclonal antibodies alone or in mix with LPS. Our unique 4-clone mixed drink has been utilized for instigating joint inflammation in different strains of mice, particularly CAIA-high responder strains, like Balb/c and DBA/1 mice.

Recently Improved 5-Clone Cocktail

Chondrex, Inc. fostered a superior 5-clone mixed drink by adding another clone to our unique 4-clone mixed drink. Three clones of this new 5-clone mixed drink perceive epitopes situated inside the LyC1 (CII 124-290) piece of CB11 (CII 124-402), while the other two clones respond with the LyC2 (CII 291-374) part. The arthritogenicity of the new 5-clone mixed drink is twice more noteworthy than our 4-clone mixed drink and a contender’s 4 clone mixed drink, in view of a high adequacy of supplement obsession.

Arthritogenicity of Chondrex 5-Clone Cocktail versus Contender

The arthritogenicity of the new 5-clone mixed drink was contrasted and the present 4-clone mixed drink and a contender’s 4-clone mixed drink in DBA/1, Balb/c, and C57BL/6 mice. DBA/1 and Balb/c mice got 1.5 mg, while C57BL/6 mice got 5 mg of every monoclonal neutralizer mixed drink, by IP on day 0 followed by an IP infusion of LPS (50 μg) on day 3.

Utilizing an equivalent portion, the new 5-clone mixed drink incited more extreme and steady joint inflammation in every one of the three strains of mice than our 4-clone mixed drink and a contender’s mixed drink (Figure 2).
Our new 5-clone mixed drink is likewise fit for inciting joint inflammation without LPS in specific circumstances and strains of mice.